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Masaryk University

Norwegian University of Science and Technology

This project has established collaboration between Masaryk University (Brno, Czech Republic) and the Norwegian University of Science and Technology (NTNU, Trondheim, Norway). Some additional selected taxonomic experts from other institutions have been associated. The project’s aims have been fulfilled and we have successfully DNA-barcoded approximately 100 taxa of bryophytes and lichens, and established a permanent workstation for DNA-barcoding at Masaryk University. Barcode sequences have been used to study species delimitation in the lichen genera Cladonia, Placynthium, and Solenopsora, and the bryophyte genus Sphagnum. This project was the first attempt to DNA-barcode bryophytes and lichens in the Czech Republic. Additional DNA-regions were produced for performing multi-locus phylogenetic analyses of the selected lichen genera while future collaboration has also been established. Moreover, we have been studying the circumarctic, boreal and temperate populations of Sphagnum warnstorfii using restriction site associated DNA markers (RADseq). The RADseq data was produced through mobilities at the NTNU University Museum, under the supervision of the local experts. The implementation of this NGS approach at Masaryk University was done and knowledge was shared successfully and will continue in the future. This project has increased the level of competence of students and staff of Masaryk University when it comes to DNA barcoding and NGS approaches to biosystematics. It has formed the basis for further collaboration in the future.

1 236 302 CZK